Sidec > Technology > Protein Tomography™
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Macromedia.comProtein Tomography™
Investigate proteins in a biological context
Protein Tomography™ is a unique method combining cryo-EM, electron tomography and advanced data-processing algorithms to perform in situ or in vitro tomography of proteins.
- Protein Tomography™ provides tomograms of individual proteins and protein complexes
- Protein Tomography™ is the only method that picture membrane proteins in a cellular context
- Protein Tomography™ results are directly comparable between in vitro and in situ analysis, and between species.
In cryo-electron microscopy, the protein samples are embedded in a thin amorphous layer of ice to preserve their ultra-structure. Electron Tomography uses a tilt-series of electron micrographs, creating 3-D images or tomograms when brought together.
The Sidec scientists use unique resolution-enhancing algorithms, developed by Professor Ulf Skoglund at the Karolinska Institute in Sweden, for data processing and 3-D reconstruction. The enhanced resolution allow us to observe proteins (even membrane proteins) and protein complexes as they interact in vitro or in situ at a fixed moment in time.
