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Protein Tomography™ allows 3-D imaging of an individual, hydrated object in solution, in contrast to x-ray crystallography, NMR and single particle electron tomography techniques. The method has been used previously to study cells and large macromolecules.
Study objective
In this academic collaboration between the Karolinska Institute, Karolinska Hospital and Huddinge University Hospital in Sweden, Protein Tomography™ was used to study the conformation and flexibility of monoclonal IgG molecules with a molecular weight of 150 kDa.
Conclusions
Individual molecules seen from several angles
The tomograms above show five individual IgG molecules (A-E), seen from four different views, which are separated by 90 degrees rotation around the vertical axis. The molecules are visualized using volume rendering, and intensities are colored from red to blue.